Protein / target

Cyclin-dependent kinase 4

CDK4P11802Homo sapiensSwiss-Prot
Clinically validated
Therapeutic maturity
5
Approved medicines
30
Clinical trials
Small-molecule tractable
Druggability
Approved Drug

Protein at a glance

Biological role

Cyclin-dependent protein serine/threonine kinase activity

Strongest disease association

melanoma, cutaneous malignant, susceptibility to, 3

Genetic evidence · score 0.80

Therapeutic maturity

Clinically validated target

5 approved medicines against this target

Druggability

Small molecule

Open Targets tractability · Approved Drug

Clinical development

5 approved · 12 in clinical development

30 linked trials

Research activity

Emerging research

10 papers · latest 2025

Derived from structured UniProt, Open Targets and literature data on this page.

Protein profile

UniProt 2026_02

Canonical identity and biological annotation from UniProt.

Function

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex

Subcellular location

CytoplasmNucleusNucleus membrane
Domains and Gene Ontology detail (29)

Domains & features

Protein kinase

Gene Ontology

  • Cbicellular tight junction
  • Cchromatin
  • Ccyclin D1-CDK4 complex
  • Ccyclin D2-CDK4 complex
  • Ccyclin-dependent protein kinase holoenzyme complex
  • Ccytoplasm
  • Ccytosol
  • Cnuclear membrane
  • Cnucleolus
  • Cnucleoplasm
  • Cnucleus
  • Ctranscription regulator complex

303 aa · 34 kDa · 2 isoforms

Biological roles

Reactome v97

What this protein does, drawn together from its UniProt function, Gene Ontology terms and Reactome pathways.

Transcriptional regulationUniProt · GO · ReactomeKinase signallingUniProt · GOCell adhesionGO
View supporting evidence

Transcriptional regulation

  • ·Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit…
  • ·transcription regulator complex
  • ·regulation of gene expression
  • ·regulation of transcription initiation by RNA polymerase II

Kinase signalling

  • ·Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit…
  • ·cyclin-dependent protein kinase holoenzyme complex
  • ·cyclin-dependent protein serine/threonine kinase activity
  • ·protein serine kinase activity

Cell adhesion

  • ·bicellular tight junction
View underlying pathways (19)

Concepts derived from UniProt GO Reactome — each badge above shows which sources supported that role.

Interaction neighbourhood

STRING v12.0

Proteins with the strongest functional or physical association. Node size and line weight reflect STRING confidence; hover or select a partner to inspect one association.

CCNL2CDKN2DRB1CCND1CDKN2ACCNA2CDKN1BCCND2CCND3CDKN1ACDK4

10 strongest partners — larger node and heavier line mean higher confidence

Functional and physical associations from STRING v12.0. Only associations with this protein are drawn — partner-to-partner links are not part of this evidence.

Drugs targeting this protein

3

Whether each drug engages this protein directly or through a complex, and how many other targets are recorded for it. Direct binders with few recorded targets are listed first.

palbociclib
Narrow target profileApprovedInhibitor

Cyclin-dependent kinase 4/cyclin D1 inhibitor

Appears in clinical studies involving breast cancer, breast carcinoma, breast neoplasm, neoplasm

Direct interaction with this protein · 1 of 3 recorded protein targets — narrow recorded profile

abemaciclib
Narrow target profileApprovedInhibitor

Cyclin-dependent kinase 4 inhibitor

Appears in clinical studies involving breast cancer, breast neoplasm, neoplasm, breast carcinoma

Direct interaction with this protein · 1 of 2 recorded protein targets — narrow recorded profile

ribociclib
Narrow target profilePhase 3Inhibitor

Cyclin-dependent kinase 4 inhibitor

Appears in clinical studies involving breast cancer, melanoma, hematopoietic and lymphoid cell neoplasm, teratoma

Direct interaction with this protein · 1 of 2 recorded protein targets — narrow recorded profile

ChEMBL mechanism, action type and target identity. Open Targets clinical status and indications.

Translational evidence

Open Targets 26

Why this target matters therapeutically — disease associations, drugs in development, tractability and safety, from Open Targets.

Strongest genetic associations

Human genetic evidence — the most direct causal link between this target and a disease.

melanoma, cutaneous malignant, susceptibility to, 30.80

Genetic · overall 0.70

familial melanoma0.80

Genetic · overall 0.58

multiple sclerosis0.03

Genetic · overall 0.53

Highest-confidence therapeutic associations

Diseases where a drug acting on this target has already reached clinical development.

breast cancer0.99

Clinical · overall 0.63

small cell lung carcinoma0.88

Clinical · overall 0.59

breast carcinoma0.87

Clinical · overall 0.63

breast neoplasm0.86

Clinical · overall 0.53

neoplasm0.84

Clinical · overall 0.54

Highest overall evidence

Remaining associations by Open Targets' aggregated evidence score.

neurodegenerative disease0.55

Pathway

Alzheimer disease0.54

Pathway

Show all associations
melanoma, cutaneous malignant, susceptibility to, 30.70
breast carcinoma0.63
breast cancer0.63
small cell lung carcinoma0.59
familial melanoma0.58
neurodegenerative disease0.55
Alzheimer disease0.54
neoplasm0.54
breast neoplasm0.53
multiple sclerosis0.53

Open Targets ranks 1,396 associations for this target and we store the top 10 by aggregated score. The long tail is largely literature co-mention and RNA-expression evidence, not evidence that this target drives those diseases.

Known drugs · 17 total

AT-7519Phase 2

mantle cell lymphoma · B-cell chronic lymphocytic leukemia · plasma cell myeloma

MILCICLIBPhase 2

thymic carcinoma · thymoma · hepatocellular carcinoma

AZD-5438Phase 1
RGB-286638Phase 1

lymphoid neoplasm

TRILACICLIB DIHYDROCHLORIDEApproval

small cell lung carcinoma

PALBOCICLIBApproval

breast cancer · breast carcinoma · breast neoplasm

PHA-793887Phase 1
ABEMACICLIBApproval

breast cancer · breast neoplasm · neoplasm

RIBOCICLIBPhase 3

breast cancer · melanoma · hematopoietic and lymphoid cell neoplasm

ALVOCIDIBPhase 3

B-cell chronic lymphocytic leukemia · acute myeloid leukemia by FAB classification · non-Hodgkin lymphoma

Tractability

SM · Approved DrugSM · Structure with LigandSM · High-Quality LigandSM · High-Quality PocketSM · Druggable FamilyPR · LiteraturePR · Database UbiquitinationPR · Half-life DataPR · Small Molecule Binder

Clinical trials

30

Trials of drugs that target this protein — reached indirectly through those drugs, so a trial listed here studies the drug, not the protein.

Show remaining trials (24)

ClinicalTrials.gov via the drug-target graph.

Research activity

10 papers · to 2025

Papers linked directly to this protein. This is the protein's own literature — descriptor-derived papers are kept separate below.

Most cited

Recent

Landscape of Baseline and Acquired Genomic Alterations in Circulating Tumor DNA with Abemaciclib Alone or with Endocrine Therapy in Advanced Breast Cancer.

Goetz MP · Clinical cancer research : an official journal of the American Association for Cancer Research · 2024

Cyclin D1-Cdk4 regulates neuronal activity through phosphorylation of GABAA receptors.

Pedraza N · Cellular and molecular life sciences : CMLS · 2023

CDK4/6-MEK Inhibition in MPNSTs Causes Plasma Cell Infiltration, Sensitization to PD-L1 Blockade, and Tumor Regression.

Kohlmeyer JL · Clinical cancer research : an official journal of the American Association for Cancer Research · 2023

Europe PMC papers linked directly to this protein.

Forefront confidence

Synthesis

Our own weighting of the evidence behind each disease association. Human genetics and clinical evidence count for most; literature co-mention counts for little, because two entities sharing an abstract is not evidence that one drives the other.

melanoma, cutaneous malignant, susceptibility to, 3Well supported
0.83
agreement 0.710.95
Genetic87%Animal model13%Literature0%Genetic literaturedup

Open Targets aggregate 0.70 · 3 independent evidence families · 1 not counted as duplicate

breast carcinomaWell supported
0.83
agreement 0.720.93
Clinical52%Somatic mutation24%Pathway13%Literature12%

Open Targets aggregate 0.63 · 4 independent evidence families

familial melanomaWell supported
0.82
agreement 0.700.94
Genetic85%Animal model13%Literature3%Genetic literaturedup

Open Targets aggregate 0.58 · 3 independent evidence families · 1 not counted as duplicate

breast cancerWell supported
0.78
agreement 0.620.93
Clinical83%Literature17%

Open Targets aggregate 0.63 · 2 independent evidence families

small cell lung carcinomaWell supported
0.76
agreement 0.640.88
Clinical69%Somatic mutation21%Literature11%

Open Targets aggregate 0.59 · 3 independent evidence families

The evidence agreement range shows how closely the independent evidence families agree — it is not a statistical confidence interval, and nothing here is fitted to outcome data. Derived from Open Targets evidence types under Forefront weighting; the underlying per-type scores are shown above so the calculation can be checked.

What's happening now

60

Recent activity around this target, drawn from one canonical event stream. Every item is reached through 3 drugs that target this protein, so each event is news about that drug rather than about the protein directly.

  1. Trial status changed2026-07-22

    Phase I Study of the Pan-ERBB Inhibitor Neratinib Given in Combination With Everolimus, Palbociclib, or Trametinib in Advanced Cancer Subjects With EGFR Mutation/Amplification, HER2 Mutation/Amplification, or HER3/4 Mutation or KRAS Mutation

    Status changed to Terminated · ClinicalTrials.gov · via palbociclib

  2. Indication expanded2026-07-13

    Indication expansion: ABEMACICLIB (NDA208716)

    fda · regulatory · fda · via abemaciclib

  3. Trial status changed2026-07-06

    PIKture-01: First-in-Human Study of the PI3KαH1047R Mutant-Selective Inhibitor OKI-219 as Monotherapy in Participants With Advanced Solid Tumors and as Part of Combination Therapy in Participants With Advanced Breast Cancer

    Status changed to Active, not recruiting · ClinicalTrials.gov · via ribociclib

  4. Indication expanded2026-07-01

    Indication expansion: RIBOCICLIB (NDA209092)

    fda · regulatory · fda · via ribociclib

  5. Indication expanded2026-06-24

    Indication expansion: PALBOCICLIB (NDA212436)

    fda · regulatory · fda · via palbociclib

  6. Indication expanded2026-06-24

    Indication expansion: PALBOCICLIB (NDA207103)

    fda · regulatory · fda · via palbociclib

  7. Regulatory approval2026-06-19

    Approval: Palbociclib Viatris (EMA)

    ema · regulatory · ema · via palbociclib

  8. Indication expanded2025-09-16

    Indication expansion: PALBOCICLIB (NDA212436)

    fda · regulatory · fda · via palbociclib

  9. Indication expanded2025-09-16

    Indication expansion: PALBOCICLIB (NDA207103)

    fda · regulatory · fda · via palbociclib

  10. Supplemental approval2025-09-16

    Supplemental approval: RIBOCICLIB (NDA209092)

    fda · regulatory · fda · via ribociclib

  11. Label change2025-07-28

    Label change: RIBOCICLIB (NDA209092)

    fda · regulatory · fda · via ribociclib

  12. Indication expanded2025-04-23

    Indication expansion: PALBOCICLIB (NDA212436)

    fda · regulatory · fda · via palbociclib

Objective event titles are shown unmodified; the event kind and significance line are derived from structured fields. Forefront AttentionEvent stream aggregating Europe PMC Regulatory filings ClinicalTrials.gov.