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Protein / target

Tumor necrosis factor

Encoded byTNFP01375Homo sapiensSwiss-Prot
Clinically validated
Therapeutic maturity
7
Approved medicines
Open Targets target-level
View by indication →
30
Clinical trials
Small-molecule tractable
Druggability
Structure with Ligand

Protein at a glance

Biological role

Transcription cis-regulatory region binding

Strongest disease association

Arthritis, Psoriatic

Via encoding gene TNF · Genetic literature evidence · score 0.61

Therapeutic position

Established drug target

Antibodies

Research activity

Actively researched

63 papers · latest 2025

Derived from structured UniProt, Open Targets and literature data on this page.

Protein profile

UniProt 2026_02

Canonical identity and biological annotation from UniProt.

Function overview

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.

View complete UniProt function annotation

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line (PubMed:16829952, PubMed:22517918, PubMed:23396208). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (PubMed:12794819). Promotes osteoclastogenesis and therefore mediates bone resorption (By similarity)

Subcellular location

Cell membraneMembraneSecreted
Domains and Gene Ontology detail (165)

Domains & features

THD

Gene Ontology

  • Ccell surface
  • Cexternal side of plasma membrane
  • Cextracellular region
  • Cextracellular space
  • Cmembrane raft
  • Cneuronal cell body
  • Cphagocytic cup
  • Cplasma membrane
  • Crecycling endosome
  • Fcytokine activity
  • Fhistone H3K9ac reader activity
  • Fidentical protein binding

233 aa · 26 kDa

Biological roles

Reactome v97

What this protein does, drawn together from its UniProt function, Gene Ontology terms and Reactome pathways.

Cell migrationGOExcitatory neurotransmissionGOCell-cycle regulationGOImmune signallingUniProt · GO · ReactomeTranscriptional regulationGO · ReactomeApoptosis & cell deathGO
View supporting evidence

Cell migration

  • ·negative regulation of blood vessel endothelial cell migration
  • ·positive regulation of mononuclear cell migration

Excitatory neurotransmission

  • ·regulation of synaptic transmission, glutamatergic

Cell-cycle regulation

  • ·negative regulation of mitotic cell cycle

Immune signalling

  • ·Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macro…
  • ·cytokine activity
  • ·antiviral innate immune response
  • ·chronic inflammatory response to antigenic stimulus

Transcriptional regulation

  • ·transcription cis-regulatory region binding
  • ·negative regulation of DNA-templated transcription
  • ·negative regulation of gene expression
  • ·negative regulation of miRNA transcription

Apoptosis & cell death

  • ·endothelial cell apoptotic process
  • ·positive regulation of apoptotic process
  • ·positive regulation of neuron apoptotic process
  • ·positive regulation of programmed cell death
View underlying pathways (11)

Concepts derived from UniProt GO Reactome — each badge above shows which sources supported that role.

Interaction neighbourhood

STRING v12.0

Proteins with the strongest functional or physical association. Node size and line weight reflect STRING confidence; hover or select a partner to inspect one association.

TAB2NFKB1TRAF2RIPK1CHUKTRADDIKBKBBIRC2CASP8MAP3K7TNF

10 strongest partners — larger node and heavier line mean higher confidence

Functional and physical associations from STRING v12.0. Only associations with this protein are drawn — partner-to-partner links are not part of this evidence.

Approved medicines with mapped indications

5 medicines · 12 areas

Approved therapies targeting this protein, grouped by what they are approved to treat — the disease-first view of the medicines below. Relationships come from the canonical approved-indication graph (ChEMBL phase-4), the same source as the drug cards.

Colitis, Ulcerative3 medicines
Crohn's Disease3 medicines
Psoriasis3 medicines
Arthritis, Juvenile2 medicines
Arthritis1 medicine
Hidradenitis Suppurativa1 medicine
Inflammation1 medicine
Uveitis1 medicine

7 medicines meet Open Targets' target-level approved-medicine definition; the 5 shown here are those on this page with a canonical approved disease indication in the graph. Approved indications from ChEMBL (phase-4), via the canonical drug→disease graph.

Drugs targeting this protein

5

How approved and investigational drugs engage this protein — mechanism and action type, direct vs complex targeting, and how broadly each acts across other targets. A drug-first view (the section above groups the approved ones disease-first); direct binders with few recorded targets are listed first.

golimumab
Narrow target profileApprovedInhibitor

TNF-alpha inhibitor

Indicated for Arthritis, Psoriatic, Arthritis, Rheumatoid, Colitis, Ulcerative, Immune System Diseases

Direct interaction with this protein · Only this protein recorded as a target

Etanercept
Narrow target profileApprovedInhibitor

TNF-alpha inhibitor

Indicated for Arthritis, Juvenile, Arthritis, Psoriatic, Arthritis, Rheumatoid, Immune System Diseases

Direct interaction with this protein · Only this protein recorded as a target

Adalimumab
Narrow target profileApprovedInhibitor

TNF-alpha inhibitor

Indicated for Arthritis, Arthritis, Juvenile, Arthritis, Psoriatic, Arthritis, Rheumatoid

Direct interaction with this protein · Only this protein recorded as a target

Infliximab
Narrow target profileApprovedInhibitor

TNF-alpha inhibitor

Indicated for Arthritis, Psoriatic, Arthritis, Rheumatoid, Colitis, Ulcerative, Crohn's Disease

Direct interaction with this protein · Only this protein recorded as a target

certolizumab pegol
Narrow target profileApprovedInhibitor

TNF-alpha inhibitor

Indicated for Arthritis, Psoriatic, Arthritis, Rheumatoid, Crohn's Disease, Immune System Diseases

Direct interaction with this protein · Only this protein recorded as a target

ChEMBL mechanism, action type, target identity and approved indications. Open Targets clinical status.

Translational evidence

Open Targets 26

Why this target matters therapeutically, strongest evidence first. Disease associations are gene-level (via the gene that encodes this protein) and open into the full confidence synthesis; the development universe, tractability and safety annotations are target-level, from Open Targets.

Strongest disease associations · via encoding gene TNF

Gene-level evidence surfaced through the gene TNFthat encodes this protein — not a direct protein–disease relationship. Ranked by Forefront's causal-directness weighting, so genetically- and clinically-evidenced diseases lead over ones that merely share the literature.

Arthritis, Psoriatic
0.88Well supported

Clinical evidence dominant · Open Targets 0.70

Psoriasis
0.81Well supported

Clinical evidence dominant · Open Targets 0.64

Arthritis, Rheumatoid
0.79Well supported

Clinical evidence dominant · Open Targets 0.64

Colitis, Ulcerative
0.78Well supported

Clinical evidence dominant · Open Targets 0.63

Arthritis, Juvenile
0.77Well supported

Clinical evidence dominant · Open Targets 0.63

View evidence synthesis (5)
Arthritis, PsoriaticWell supported
0.88
agreement 0.770.98
Clinical57%Genetic literature37%Literature6%RNA expression0%

Open Targets aggregate 0.70 · 4 independent evidence families

PsoriasisWell supported
0.81
agreement 0.680.94
Clinical74%Literature15%Animal model12%

Open Targets aggregate 0.64 · 3 independent evidence families

Arthritis, RheumatoidWell supported
0.79
agreement 0.630.94
Clinical83%Literature17%

Open Targets aggregate 0.64 · 2 independent evidence families

Colitis, UlcerativeWell supported
0.78
agreement 0.640.91
Clinical86%Literature14%RNA expression1%

Open Targets aggregate 0.63 · 3 independent evidence families

Arthritis, JuvenileWell supported
0.77
agreement 0.620.93
Clinical84%Literature17%

Open Targets aggregate 0.63 · 2 independent evidence families

The evidence agreement range shows how closely the independent evidence families agree — it is not a statistical confidence interval, and nothing here is fitted to outcome data. Derived from Open Targets evidence types under Forefront weighting; the per-type scores above show the calculation.

Show all associations
Arthritis, Psoriatic0.70
Psoriasis0.64
Arthritis, Rheumatoid0.64
Colitis, Ulcerative0.63
Arthritis, Juvenile0.63
Spondylitis, Ankylosing0.62
Crohn's Disease0.62
Psoriasis vulgaris0.61
Uveitis0.61
Hidradenitis Suppurativa0.60

Drug development

14 compounds recorded · 7 approved · 7 in clinical development

Open Targets' development universe — every compound recorded against the target at any stage, not all approved medicines. Distinct from the 5 drugs that target this protein in Forefront's canonical graph (5 with a mapped approved indication, shown above): these count different sets and are not a subset relation.

View all recorded compounds (10)
ETANERCEPTApproval
PLACULUMABPhase 2
INFLIXIMABApproval
ONERCEPTPhase 3
AFELIMOMABApproval
REMTOLUMABPhase 2
ABBV-3373Phase 2
LENERCEPTPhase 3
OZORALIZUMABApproval
NERELIMOMABPhase 3

Open Targets known-drugs universe. Drug name and highest clinical stage only — the disease relationship is NOT read from this slice (it carries trial-context noise); approved indications come from the canonical graph above.

Tractability

Small moleculesEmerging

Feasibility evidence (structure with ligand and high-quality ligand) — no clinical-stage drug of this modality recorded.

AntibodiesStrong

Approved Drug and UniProt loc high conf support this modality.

Protein degradersEmerging

Feasibility evidence (small molecule binder) — no clinical-stage drug of this modality recorded.

Other modalitiesStrong

Approved Drug support this modality.

View underlying tractability evidence (11)
SM · Structure with LigandSM · High-Quality LigandSM · High-Quality PocketSM · Druggable FamilyAB · Approved DrugAB · UniProt loc high confAB · GO CC high confAB · UniProt loc med confAB · UniProt SigP or TMHMMPR · Small Molecule BinderOC · Approved Drug

Raw Open Targets tractability assessment buckets, by modality.

Safety-related annotations

hand-foot syndromeClinPGxhypersensitivityClinPGxthrombocytopeniaClinPGxToxic liver diseaseClinPGxinsufficient dataClinPGxchance of severe hypersensitivityClinPGxchance of severe hypersensitivity to carbamazepine treatmentClinPGxregulation of gene expressionToxCastneuropathyClinPGx

Terms indexed against this target in Open Targets' safety data, with their datasource. These are annotations, not causal claims: the direction of effect (whether activation or inhibition is implicated), species and evidence strength are not captured here, so an entry does not mean that modulating this target is known to cause that condition.

Clinical trials

30

Trials of drugs that target this protein — reached indirectly through those drugs, so a trial listed here studies the drug, not the protein. Ranked by active status, then clinical phase and recency, and spread across the targeting drugs.

View all trials (26)

RECRUITING · via Infliximab · NCT02638701

RECRUITING · via Adalimumab · NCT06062875

ClinicalTrials.gov via the drug-target graph.

What's happening now

12

Recent therapeutic activity around this target — regulatory actions, clinical trials and safety signals for 5 drugs that target this protein, plus publications where such a drug is a genuine subject. Every item is reached indirectly through the drug, not the protein itself; incidental mentions (a paper that merely measures a drug) and press items are excluded.

  1. Withdrawn from market2026-05-21

    Market withdrawal: Inflectra (EMA)

    ema · market · ema · via Infliximab

  2. Regulatory approval2026-02-10

    Approval: Gotenfia (EMA)

    ema · regulatory · ema · via golimumab

  3. Regulatory approval2025-11-17

    Approval: Gobivaz (EMA)

    ema · regulatory · ema · via golimumab

  4. New publication2025-04-18
    Divergent disruptive effects of soluble recombinant tau assemblies on synaptic plasticity in vivo.

    Molecular brain · 2025 · 3 citations · Europe PMC · via Etanercept

  5. New publication2024-08-28
    Neurological Side Effects of TNF-α Inhibitors Revisited: A Review of Case Reports.

    Medicina (Kaunas, Lithuania) · 2024 · 12 citations · Europe PMC · via Adalimumab

  6. New publication2023-11-01
    Efficacy and safety of golimumab in patients with non-radiographic axial spondyloarthritis: a withdrawal and retreatment study (GO-BACK).

    Rheumatology (Oxford, England) · 2023 · 8 citations · Europe PMC · via golimumab

  7. New publication2022-03-29
    New and Emerging Targeted Therapies for Hidradenitis Suppurativa.

    International journal of molecular sciences · 2022 · 46 citations · Europe PMC · via Adalimumab

  8. New publication2022-03-01
    Long-term safety and clinical outcomes of certolizumab pegol treatment in patients with active non-radiographic axial spondyloarthritis: 3-year results from the phase 3 C-axSpAnd study.

    RMD open · 2022 · 8 citations · Europe PMC · via certolizumab pegol

  9. Regulatory approval2021-11-15

    Approval: Hukyndra (EMA)

    ema · regulatory · ema · via Adalimumab

  10. Regulatory approval2021-11-12

    Approval: Libmyris (EMA)

    ema · regulatory · ema · via Adalimumab

  11. New publication2021-08-17
    Three-year efficacy and safety of certolizumab pegol for the treatment of plaque psoriasis: results from the randomized phase 3 CIMPACT trial.

    Journal of the European Academy of Dermatology and Venereology : JEADV · 2021 · 8 citations · Europe PMC · via certolizumab pegol

  12. Regulatory approval2021-02-11

    Approval: Yuflyma (EMA)

    ema · regulatory · ema · via Adalimumab

Objective event titles are shown unmodified; the event kind and significance line are derived from structured fields. Forefront AttentionEvent stream aggregating Europe PMC Regulatory filings ClinicalTrials.gov.

Research activity

63 papers · to 2025

Papers linked directly to this protein. This is the protein's own literature — descriptor-derived papers are kept separate below.

Most cited

Keane J · The New England journal of medicine · 2001

Heinrich PC · The Biochemical journal · 1990

Frangogiannis NG · Cardiovascular research · 2002

Landskron G · Journal of immunology research · 2014

Recent

Europe PMC papers linked directly to this protein.