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Protein / target

Solute carrier family 12 member 3

Encoded bySLC12A3P55017Homo sapiensSwiss-Prot
Clinically validated
Therapeutic maturity
13
Approved medicines
Open Targets target-level
View by indication →
30
Clinical trials
Small-molecule tractable
Druggability
Approved Drug

Protein at a glance

Biological role

Sodium:potassium:chloride symporter

Strongest disease association

Gitelman Syndrome

Via encoding gene SLC12A3 · Genetic evidence · score 0.95

Therapeutic position

Established drug target

Small molecules

Derived from structured UniProt, Open Targets and literature data on this page.

Protein profile

UniProt 2026_02

Canonical identity and biological annotation from UniProt.

Function overview

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules.

View complete UniProt function annotation

Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed:18270262, PubMed:21613606, PubMed:22009145, PubMed:36351028, PubMed:36792826). Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2 (By similarity). May act either independently of IL18R1, or in a complex with IL18R1 (By similarity)

Subcellular location

Cell membraneApical cell membrane
Domains and Gene Ontology detail (19)

Gene Ontology

  • Capical plasma membrane
  • Ccytosol
  • Cextracellular exosome
  • Cmembrane
  • Cplasma membrane
  • FATP binding
  • Fsodium:chloride symporter activity
  • Fsodium:potassium:chloride symporter activity
  • Pcell volume homeostasis
  • Pchloride ion homeostasis
  • Pchloride transmembrane transport
  • Ppotassium ion homeostasis

1021 aa · 113 kDa · 3 isoforms

Biological roles

What this protein does, drawn together from its UniProt function, Gene Ontology terms and Reactome pathways.

Chloride transportUniProt · GOIon channel gatingGO
View supporting evidence

Chloride transport

  • ·Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of so…
  • ·chloride ion homeostasis

Ion channel gating

  • ·sodium ion transmembrane transport

Concepts derived from UniProt GO Reactome — each badge above shows which sources supported that role.

Approved medicines with mapped indications

1 medicine · 9 areas

Approved therapies targeting this protein, grouped by what they are approved to treat — the disease-first view of the medicines below. Relationships come from the canonical approved-indication graph (ChEMBL phase-4), the same source as the drug cards.

Angina Pectoris1 medicine
Edema1 medicine
Heart Failure1 medicine
Hypertension1 medicine
Kidney Failure, Chronic1 medicine
Liver Cirrhosis1 medicine
Myocardial Infarction1 medicine
Nephrotic Syndrome1 medicine
Broader indication categories (1)
Cardiovascular Diseases1 medicine

Broad umbrella indications (e.g. “Neoplasms”). Shown here because every medicine also appears under a more specific disease above — kept for completeness, de-emphasised for clarity.

13 medicines meet Open Targets' target-level approved-medicine definition; the 1 shown here are those on this page with a canonical approved disease indication in the graph. Approved indications from ChEMBL (phase-4), via the canonical drug→disease graph.

Drugs targeting this protein

1

How approved and investigational drugs engage this protein — mechanism and action type, direct vs complex targeting, and how broadly each acts across other targets. A drug-first view (the section above groups the approved ones disease-first); direct binders with few recorded targets are listed first.

hydrochlorothiazide
Narrow target profileApprovedInhibitor

Thiazide-sensitive sodium-chloride cotransporter inhibitor

Indicated for Angina Pectoris, Edema, Heart Failure, Hypertension

Direct interaction with this protein · Only this protein recorded as a target

ChEMBL mechanism, action type, target identity and approved indications. Open Targets clinical status.

Translational evidence

Open Targets 26

Why this target matters therapeutically, strongest evidence first. Disease associations are gene-level (via the gene that encodes this protein) and open into the full confidence synthesis; the development universe, tractability and safety annotations are target-level, from Open Targets.

Strongest disease associations · via encoding gene SLC12A3

Gene-level evidence surfaced through the gene SLC12A3 that encodes this protein — not a direct protein–disease relationship. Ranked by Forefront's causal-directness weighting, so genetically- and clinically-evidenced diseases lead over ones that merely share the literature.

Gitelman Syndrome
0.97Well supported

Genetic evidence dominant · Open Targets 0.85

Hypertension
0.85Well supported

Clinical evidence dominant · Open Targets 0.67

Nephrotic Syndrome
0.82Well supported

Clinical evidence dominant · Open Targets 0.63

Kidney Failure, Chronic
0.76Well supported

Clinical evidence dominant · Open Targets 0.61

Heart Failure
0.75Moderately supported

Clinical evidence dominant · Open Targets 0.61

View evidence synthesis (5)
Gitelman SyndromeWell supported
0.97
agreement 0.851.00
Genetic76%Pathway19%Literature5%Genetic literaturedup

Open Targets aggregate 0.85 · 3 independent evidence families · 1 not counted as duplicate

HypertensionWell supported
0.85
agreement 0.740.95
Clinical64%Genetic31%Literature5%

Open Targets aggregate 0.67 · 3 independent evidence families

Nephrotic SyndromeWell supported
0.82
agreement 0.690.94
Clinical73%Animal model27%Literature0%

Open Targets aggregate 0.63 · 3 independent evidence families

Kidney Failure, ChronicWell supported
0.76
agreement 0.630.90
Clinical92%Literature5%RNA expression3%

Open Targets aggregate 0.61 · 3 independent evidence families

Heart FailureModerately supported
0.75
agreement 0.590.90
Clinical99%Literature1%

Open Targets aggregate 0.61 · 2 independent evidence families

The evidence agreement range shows how closely the independent evidence families agree — it is not a statistical confidence interval, and nothing here is fitted to outcome data. Derived from Open Targets evidence types under Forefront weighting; the per-type scores above show the calculation.

Show all associations
Gitelman Syndrome0.85
Hypertension0.67
Nephrotic Syndrome0.63
Kidney Failure, Chronic0.61
Heart Failure0.61
Myocardial Infarction0.60
Cardiovascular Diseases0.59

Drug development

13 compounds recorded · 13 approved

Open Targets' development universe — every compound recorded against the target at any stage, not all approved medicines. Distinct from the 1 drug that targets this protein in Forefront's canonical graph (1 with a mapped approved indication, shown above): these count different sets and are not a subset relation.

View all recorded compounds (10)
TRICHLORMETHIAZIDEApproval
CHLOROTHIAZIDEApproval
BENZTHIAZIDEApproval
CHLORTHALIDONEApproval
INDAPAMIDEApproval
CYCLOTHIAZIDEApproval
HYDROCHLOROTHIAZIDEApproval
HYDROFLUMETHIAZIDEApproval
QUINETHAZONEApproval
METHYCLOTHIAZIDEApproval

Open Targets known-drugs universe. Drug name and highest clinical stage only — the disease relationship is NOT read from this slice (it carries trial-context noise); approved indications come from the canonical graph above.

Tractability

Small moleculesStrong

Approved Drug and Structure with Ligand support this modality.

AntibodiesEmerging

Feasibility evidence (uniprot loc high conf and go cc high conf) — no clinical-stage drug of this modality recorded.

Protein degradersEmerging

Feasibility evidence (uniprot ubiquitination and database ubiquitination) — no clinical-stage drug of this modality recorded.

View underlying tractability evidence (9)
SM · Approved DrugSM · Structure with LigandSM · Druggable FamilyAB · UniProt loc high confAB · GO CC high confAB · UniProt loc med confAB · UniProt SigP or TMHMMPR · UniProt UbiquitinationPR · Database Ubiquitination

Raw Open Targets tractability assessment buckets, by modality.

Clinical trials

30

Trials of drugs that target this protein — reached indirectly through those drugs, so a trial listed here studies the drug, not the protein. Ranked by active status, then clinical phase and recency, and spread across the targeting drugs.

View all trials (26)

RECRUITING · via hydrochlorothiazide · NCT04934228

RECRUITING · via hydrochlorothiazide · NCT07020104

UNKNOWN · via hydrochlorothiazide · NCT05917275

COMPLETED · via hydrochlorothiazide · NCT03461003

COMPLETED · via hydrochlorothiazide · NCT02875886

UNKNOWN · via hydrochlorothiazide · NCT02100462

ClinicalTrials.gov via the drug-target graph.

What's happening now

12

Recent therapeutic activity around this target — regulatory actions, clinical trials and safety signals for a drug that targets this protein, plus publications where such a drug is a genuine subject. Every item is reached indirectly through the drug, not the protein itself; incidental mentions (a paper that merely measures a drug) and press items are excluded.

  1. Label change2026-07-24

    Label change: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  2. Label change2023-12-22

    Label change: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  3. New publication2023-03-01
    Hydrochlorothiazide and Prevention of Kidney-Stone Recurrence.

    The New England journal of medicine · 2023 · 94 citations · Europe PMC · via hydrochlorothiazide

  4. Safety communication2018-11-14

    Drug Safety Update: Hydrochlorothiazide: risk of non-melanoma skin cancer, particularly in long-term use

    mhra · safety · mhra · via hydrochlorothiazide

  5. Supplemental approval2015-07-27

    Supplemental approval: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  6. Supplemental approval2014-06-12

    Supplemental approval: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  7. New publication2013-02-12
    Genome-wide association analyses suggest NELL1 influences adverse metabolic response to HCTZ in African Americans.

    The pharmacogenomics journal · 2014 · 36 citations · Europe PMC · via hydrochlorothiazide

  8. New publication2011-11-17
    Blood pressure responses and metabolic effects of hydrochlorothiazide and atenolol.

    American journal of hypertension · 2012 · 10 citations · Europe PMC · via hydrochlorothiazide

  9. New publication2010-01-01
    Comparison of office, ambulatory, and home blood pressure antihypertensive response to atenolol and hydrochlorthiazide.

    Journal of clinical hypertension (Greenwich, Conn.) · 2010 · 9 citations · Europe PMC · via hydrochlorothiazide

  10. Supplemental approval1996-10-09

    Supplemental approval: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  11. Supplemental approval1993-11-24

    Supplemental approval: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

  12. Regulatory approval1993-03-26

    Approval: BISOPROLOL FUMARATE AND HYDROCHLOROTHIAZIDE (NDA020186)

    fda · regulatory · fda · via hydrochlorothiazide

Objective event titles are shown unmodified; the event kind and significance line are derived from structured fields. Forefront AttentionEvent stream aggregating Europe PMC Regulatory filings ClinicalTrials.gov.